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Kawai, Kiyoshi*; Sogabe, Tomochika*; Nakagawa, Hiroshi; Yamada, Takeshi*; Koseki, Shigenobu*
Journal of Food Engineering, 375, p.112066_1 - 112066_9, 2024/08
The purpose of this study was to clarify effect of glycerol and glucose on the mechanical glass transition and dynamical transition of freeze-dried bacteria at various water activity (aw)-conditions. From the water sorption isotherm, it was noted that the water content at each aw and monolayer water content were higher in the order of glycerol, glucose, non-added samples. Effect of temperature on the mean square displacement (MSD) of atoms in the samples was investigated by incoherent elastic neutron scattering. The MSD increased gradually with an increase in temperature depending on the aw and added solute. From the linear fitting, three dynamical transition temperatures (low-, middle- and high-Tds) were determined. The Couchman-Karasz model suggested that the added solute and bacteria were not completely miscible.
Kumada, Takayuki; Nakagawa, Hiroshi; Miura, Daisuke; Sekine, Yurina; Motokawa, Ryuhei; Hiroi, Kosuke; Inamura, Yasuhiro; Oku, Takayuki; Oishi, Kazuki*; Morikawa, Toshiaki*; et al.
Hamon, 34(2), p.50 - 53, 2024/05
Spin-contrast-variation (SCV) small-angle neutron scattering (SANS) enabled us to determine structure of nano-ice crystals that were generated in rapidly frozen sugar solution. In the frozen glucose solution, we found that the nano-ice crystals formed a planar structure with a radius larger than several tens of nanometers and a thickness of 2-3 nm, which was close to the critical nucleation size of ice crystals in supercooled water. This result suggests that the glucose molecules were preferentially bound to a specific face of nano-ice crystals, and then blocked the crystal growth perpendicular to that face.
Kumada, Takayuki; Nakagawa, Hiroshi; Miura, Daisuke; Sekine, Yurina; Motokawa, Ryuhei; Hiroi, Kosuke; Inamura, Yasuhiro; Oku, Takayuki; Oishi, Kazuki*; Morikawa, Toshiaki*; et al.
Journal of Physical Chemistry Letters (Internet), 14(34), p.7638 - 7643, 2023/08
Times Cited Count：0 Percentile：0.01(Chemistry, Physical)The structure of nano-ice crystals in rapidly frozen glucose solution was elucidated by using spin-contrast-variation small-angle neutron scattering, which distinguishes the nano-ice crystal signal from the frozen amorphous solution signal by the polarization-dependent neutron scattering. The analysis revealed that the nano-ice crystals form a planar structure with a diameter exceeding tens of nanometers and a thickness of 1 nm, which is close to the critical nucleation size. This result suggests that the glucose molecules are preferentially bound to a specific face of nano-ice crystals, and then block the crystal growth perpendicular to that face.
Sogabe, Tomochika*; Nakagawa, Hiroshi; Yamada, Takeshi*; Koseki, Shigenobu*; Kawai, Kiyoshi*
Biophysical Journal, 121(20), p.3874 - 3882, 2022/10
Times Cited Count：2 Percentile：34.79(Biophysics)The purpose of this study was to clarify the glass transition behavior of bacteria (
) as a function of water activity (
). Mechanical relaxation was investigated at 298 K, and the mechanical 
( at which mechanical glass transition occurs at 298 K) was determined to be 0.667. Temperature-dependency of mean square displacement was investigated by inelastic neutron scattering. From the linear fitting, two dynamical transition temperatures (low and high-
) were determined. There was a minor effect of on the low- except for the anhydrous sample. The high- largely increased with the decrease in . The dynamical determined by high- (0.688) was slightly higher than the mechanical because of the difference in the measurement time-scale. The high- was converted to the glass transition temperature (
), and anhydrous was estimated to be 411 K. Bacterial inactive-active transition was discussed according to the glass transition behavior.
Wadachi, Hiroki*; Kunieda, Takekazu*; Sakashita, Tetsuya; Kawai, Kiyoshi*; Iwata, Kenichi*; Nakahara, Yuichi*; Hamada, Nobuyuki*; Koseki, Shigenobu*; Yamamoto, Kazutaka*; Kobayashi, Yasuhiko; et al.
no journal, ,
We studied the effects of the exposure of extreme environments on life-time and breeding ability of tardigrade 
to reveal the possibility of multi-cellular organism existences in the outerspace. A life time of animals is the shortest in the heavy-ion exposed animals than any other stressors. There were significant decrease in the number of egg-laying and hatching rate between irradiated and non-irradiated animals. However, we found the next generation in all experimental conditions. Our findings suggest that tardigrades exposed to even any extreme environments could have a descendant.
; A Model for astrobiological studiesHorikawa, Daiki*; Kunieda, Takekazu*; Abe, Wataru*; Koshikawa, Shigeyuki*; Nakahara, Yuichi*; Watanabe, Masahiko*; Iwata, Kenichi*; Sakashita, Tetsuya; Hamada, Nobuyuki*; Higashi, Seigo*; et al.
no journal, ,
We report the first successful rearing of the herbivorous tardigrade, , by supplying a green alga 
as a food. The reared individuals of this species had an anhydrobiotic capacity throughout their life cycle, from eggs, to juveniles, and to adults. Reared adults, while in an anhydrobiotic state, were tolerant to temperatures -196
C and 100C. Furthermore, they were shown to be tolerant to the exposure to 99.8% acetonitrile, 1 GPa of hydrostatic pressure, or 5000 Gy of He ion radiation. We will report the details of these results, along with the description of their life history. Due to the observed tolerance to such extreme environmental conditions, we propose R. varieornatus to be included as a suitable model for astrobiological studies of multicellular organisms.
Kumada, Takayuki; Nakagawa, Hiroshi; Sekine, Yurina; Motokawa, Ryuhei; Kawai, Kiyoshi*
no journal, ,
We succeeded in observing spin-contrast-variation neutron small-angle scattering (SCV-SANS) signal from planar nano-ice crystals generated in rapidly frozen sugar solutions.
Kumada, Takayuki; Nakagawa, Hiroshi; Miura, Daisuke*; Sekine, Yurina; Motokawa, Ryuhei; Hiroi, Kosuke; Inamura, Yasuhiro; Oku, Takayuki; Oishi, Kazuki*; Morikawa, Toshiaki*; et al.
no journal, ,
The structure of nano-ice crystals in rapidly frozen sugar solution was elucidated by using spin-contrast-variation small-angle neutron scattering, which distinguishes the nano-ice crystal signal from the frozen amorphous solution signal by the polarization-dependent neutron scattering. The analysis revealed that the nano-ice crystals form a planar structure with a diameter exceeding tens of nanometers and a thickness of 1 nm, which is close to the critical nucleation size. This result suggests that the sugar molecules are preferentially bound to a specific face of nano-ice crystals, and then block the crystal growth perpendicular to that face.
