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Real-time [$$^{11}$$C]methionine translocation in barley in relation to mugineic acid phytosiderophore biosynthesis

オオムギでのムギネ酸生合成に関する[$$^{11}$$C]メチオニンの実時間での転流

Bughio, N.*; 中西 啓仁*; 清宮 正一郎*; 松橋 信平; 石岡 典子; 渡辺 智; 内田 博*; 辻 淳憲*; 長 明彦 ; 久米 民和; 橋本 昭司; 関根 俊明; 森 敏*

Bughio, N.*; Nakanishi, Hiromi*; Kiyomiya, Shoichiro*; Matsuhashi, Shimpei; Ishioka, Noriko; Watanabe, Satoshi; Uchida, Hiroshi*; Tsuji, Atsunori*; Osa, Akihiko; Kume, Tamikazu; Hashimoto, Shoji; Sekine, Toshiaki; Mori, Satoshi*

オオムギが土壌中の鉄を獲得するために分泌するムギネ酸の前駆体であるメチオニンが、植物体のどこに起源をもつかを明らかにするために、リアルタイムでの[$$^{11}$$C]メチオニン転流をポジトロンイメージング法を中心とした計測により行なった。外部から供給した[$$^{11}$$C]メチオニンが鉄欠乏オオムギの根に保持され、ムギネ酸生合成に使われたこと、地上部から根へのメチオニンの移行が見られなかったことなどから、ムギネ酸の前駆体であるメチオニンは植物体の根に起源をもつと結論した。

[$$^{11}$$C]Methionine ([$$^{11}$$C]Met) was supplied through barley roots and the $$^{11}$$C signal was follwoed using a real-time imaging system (PETIS), with subsequent development of autoradiographic images of the whole plant. In all cases, [$$^{11}$$C]Met was first translocated to the discrimination center, and this part was strongly labeled. Met absorbed by roots of the plants was subsequently translocated to other parts of the plant. In Fe-deficient (-Fe) barley plants, a drastic reduction in [$$^{11}$$C]Met translocation from the roots to the shoot was observed, while a greater amount of $$^{11}$$C was found in the leaves of Fe-sufficient or Met-pretreated -Fe plants. Treatment of -Fe plants with amiooxyacetic acid increased the translocation of [$$^{11}$$C]Met to the shoot. The retention of exogenously supplied [$$^{11}$$C]Met in the roots of -Fe barley indicates that the Met is used in the biosynthesis of mugineic acid (MA) in barley roots. This and the absence of Met movement from shoots to the roots suggest that the MA phytosiderophores precursor Met originates in the roots of plants.

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パーセンタイル:39.17

分野:Plant Sciences

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