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Disruption of the ${it AtREV3}$ gene causes hypersensitivity to Ultraviolet B light and $$gamma$$-rays in Arabidopsis; Implication of the presence of a translesion synthesis mechanism in plants

Sakamoto, Ayako; Lan, V. T. T.; Hase, Yoshihiro; Shikazono, Naoya; Matsunaga, Tsukasa*; Tanaka, Atsushi

In order to investigate UV-response mechanisms in higher plants, we isolated a UV-sensitive mutant, ${it rev3-1}$, in ${it Arabidopsis}$. The root growth of ${it rev3-1}$ was inhibited after UV-B irradiation under both photoreactivating and non-photoreactivating conditions. We found that chromosome 1 of ${it rev3-1}$ was broken at least at three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase $$zeta$$ (${it AtREV3}$), which is thought to be involved in translesion synthesis. The ${it rev3-1}$ seedlings were also sensitive to $$gamma$$-rays and MMC, which are known to inhibit DNA replication. The incorporation of BrdU after UV-B irradiation was less in ${it suv1}$ than in the wild type plant. These results suggest that UV-damaged DNA interrupted DNA replication in the ${it rev3-1}$ mutant, leading to the inhibition of cell division and root elongation.

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Category:Biochemistry & Molecular Biology

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