Operation manual of Microbeam System in Takasaki for Biological Application (MiST-BA)

Sakashita, Tetsuya; Yokota, Yuichiro; Wada, Seiichi; Funayama, Tomoo; Kobayashi, Yasuhiko

Microbeam System is a powerful tool for micro-radiosurgery studies and direct investigation of cell-to-cell communications such as "bystander effects". Microbeam system in Takasaki for biological application (MiST-BA) has been developed for several years and applied to some cases. There were fate mapping of the cellular blastoderm stage egg of the silkworm and bystander effects such as inhibition of cell proliferation, induction of micronuclei, and so on. The aim of this report (operation manual) is to provide a simple and easy usage of MiST-BA for current and new users. MiST-BA consists of three parts; (1) Offline microscope control system for cell-finding, (2) Online microscope control system for cell-targeting and irradiating, and (3) Beam shutter control system for cell irradiation with a precise number of heavy ions. The report presents the outline of MiST-BA, the operation protocol of each part, examples of a microbeam irradiation experiment using CHO-K1 cells, silkworm eggs, and Tobacco protoplast cells, and Trouble shooting.