Roles of arabidopsis and in translation synthesis

Takahashi, Shinya; Sakamoto, Ayako; Sato, Shusei*; Kato, Tomohiko*; Tabata, Satoshi*; Tanaka, Atsushi

The error-prone DNA translation synthesis (error-prone TLS) has been well characterized in yeast and mammalians, but not in higher plants. Recent finding of an -disrupted mutant () in Arabidopsis suggested that the error-prone TLS is significant for tolerance to DNA damages in higher plant. To clarify the details of the error-prone TLS in higher plants, we analyzed the T-DNA inserted Arabidopsis mutants defective in or genes, which are thought to be involved in the error-prone TLS system. The mutant () was sensitive to UV-B and cisplatin. The mutant () was sensitive to long-term UV-B and cisplatin. These results suggest TLS mechanism exists in a higher plant and show that and have important roles in tolerating exposure to DNA-damaging agents, but their function might be redundant.