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A Method to quantitate rate constant of photoassimilation within leaf using Carbon-11-labeled Carbon dioxide and positron emitting tracer imaging system

Kawachi, Naoki; Ishii, Satomi; Sakamoto, Koichi; Fujimaki, Shu; Suzui, Nobuo; Ishioka, Noriko; Matsuhashi, Shimpei

Positron Emitting Tracer Imaging System (PETIS) and Caron-11-labeled carbon dioxide ($$^{11}$$CO$$_{2}$$) could image photosynthesis in plant leaf, and $$^{11}$$C kinetics made it possible to estimate physiological function parameter in photosynthesis. With an exposure $$^{11}$$CO$$_{2}$$-gas to a leaf, together with air containing an ambient concentration of non-radioactive carrier CO$$_{2}$$ gas, PETIS experiments were performed with four light conditions on a single leaf. In order to estimate the rate constant of photosynthesis parameters, time activity curves of $$^{11}$$CO$$_{2}$$ gas input and leaf response were fitted to an appropriate compartmental tracer kinetic model, which applies of influx and efflux for photoassimilation and photosynthate delivery rate constants respectively. These results of photosynthesis parameters are consistent under light conditions.

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