Detection of oxidative DNA damage using Anti-8-OHdG Antibody; A Useful method to distinguish irradiated foods

Kikuchi, Masahiro; Mohd Dzomir, A. Z.; Gunawardane, C. R.; Alam, M. K.; Kobayashi, Yasuhiko

Ionizing radiation has been used for reducing food losses and securing food safety. The development of versatile detection methods of irradiated foods is needed for appropriate management. In an effort to distinguish between irradiated and non-irradiated meats, a detection method of oxidative DNA base damages using chemiluminescence enzyme-linked immunosorbent assay with anti-8-hydroxy-2'-deoxyguanosine antibody was developed. The procedure of this method is not so complicated, and relatively short time is needed to obtain results without special techniques. For most of all foods containing DNA, this novel method should be applicable to detect the damaged DNA of irradiated foods. Application to beef meat irradiated with 3 kGy or higher was suggested to be detectable successfully.