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Report No.
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Expression and purification of extracellular region of interleukin-13 receptor $$alpha$$1 chain and its interaction with its ligand

Kuroki, Ryota; Honjo, Eijiro; Tamada, Taro; Arima, Kazuhiko*; Izuhara, Kenji*

Interleukin-13 (IL-13) interacts with two types of receptors, the IL-13R$$alpha$$1 chain (IL-13R$$alpha$$1) and the IL-4R$$alpha$$ chain (IL-4R$$alpha$$), that transduce the IL-13 signals. In order to investigate the interaction of IL-13 with IL-13R$$alpha$$1 and IL-4R$$alpha$$, the genes coding the extracellular regions of human IL-13R$$alpha$$1 and the IL-4R$$alpha$$ containing cytokine receptor homologous region (CRH), were fused with human Fc, and expressed by silk worm. After further purification with anion-exchange chromatography, the receptors were used to investigate the ligand-receptor interaction. The size exclusion chromatography showed that association between IL-13 and IL-13Ra1 could not be observed in this condition whereas the clear association of IL-13 and IL-13Ra1 was observed after adding IL-4R$$alpha$$. It indicates that the association between IL-13 and IL-4R$$alpha$$ increase the IL-13 affinity to IL-13R$$alpha$$1.

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