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Establishment of rearing system of the extremotolerant tardigrade ${it Ramazzottius varieornatus}$

Wadachi, Hiroki*; Kunieda, Takekazu*; Abe, Wataru*; Nakahara, Yuichi*; Watanabe, Masahiko*; Sakashita, Tetsuya; Hamada, Nobuyuki*; Kobayashi, Yasuhiko; Higashi, Seigo*; Okuda, Takashi*

We collected some kinds of tardigrade to develop multi-cellular model oraganism for extremotolerant. Among four kinds of collected tardigrade, we found that a tardigrade ${it Ramazzottius varieornatus}$ at Sapporo-city feeded ${it C. vulgaris}$ and could breed. The life-time of ${it R. varieornatus}$ in an artificial culture condition is about 35 days and 5.7 days for hatching. During the whole life-time, one animal laids 8 eggs. In addition, it was observed that ${it R. varieornatus}$ could enter an ametabolic dry state called anhydrobiosis in all stages of life-time. Furthermore, we observed a high surviving-rate even if ${it R. varieornatus}$ exposed in various kinds of extreme environments.



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