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RBE of carbon beams on neurons; Approach using growth cone collapse assay

Al-Jahdari, W. S.*; Suzuki, Yoshiyuki*; Yoshida, Yukari*; Hamada, Nobuyuki*; Okamoto, Masahiko*; Noda, Shinei*; Funayama, Tomoo; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Nakano, Takashi*

At the 19th JASTRO meeting, we showed that growth cone collapse (GCC) assay can be useful in radiobiological investigations. Here, we used the GCC assay to determine RBE of carbon beams on neurons. Dorsal root ganglia (DRG) and sympathetic ganglion (SYMP) were isolated from day-8 and day-16 chick embryos. Then, neurons were exposed to doses of X-rays, or high-LET $$^{12}$$C ions. Neuronal morphological changes were examined by GCC assay. Apoptosis was examined using TUNEL assay. Irradiation induced GCC and neurite destruction in a time- and dose-dependent manner. Day-8 neurons were more radiosensitive than day-16 neurons (p=0.01). Using GCC, the linear regression revealed that the carbon beams' RBE at day-8 neurons ranged 4.2- 4.6. Whilst, at day-16 was 3.3 to 3.4. For apoptosis induction, the RBE at day-8 neurons ranged 3.7-4.1, whereas at day-16 ranged 4.2 and 3.7. Conclusions: The carbon beam was 3.3-4.6 fold more effective to induced GCC and apoptosis than X-rays Day-8 is more radiosensitive than day-16 neurons.

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