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Report No.

Analysis on mutation induced by ion-beam and $$gamma$$-rays in ${it Arabidopsis}$

Yoshihara, Ryohei; Hase, Yoshihiro; Takimoto, Koichi*; Narumi, Issei

Ionizing radiation induces DNA damages which cause mutation. Organisms have DNA repair systems to repair DNA damages. The relationship between types of mutation induced by ionizing radiation and DNA repair systems are well studied in bacteria and animal. In plant, however, little is known about it. In this study we will investigate ion-beam and $$gamma$$-rays induced mutations in plant by using ${it Arabidopsis}$ mutation detection system (Yoshihara et al. 2006). Ion-beam and $$gamma$$-rays are irradiated to ${it Arabidopsis}$ containing ${it E. coli rpsL}$ gene. Mutated ${it rpsL}$ gene is collected as a plasmid by plasmid rescue from chromosomal DNA and analyze the types of mutations. We will report the results of ion-beam and $$gamma$$-rays induced mutations detected by this system.



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