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Effects of locally targeted heavy-ion and laser microbeam on root hydrotropism in ${it Arabidopsis thaliana}$

Miyazawa, Yutaka*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Negishi, Hiroshi*; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Morohashi, Keita*; Kakizaki, Takehiko*; Wada, Seiichi*; Kobayashi, Yasuhiko; Fujii, Nobuharu*; Takahashi, Hideyuki*

We examined the role of root cap and elongation zone cells in root hydrotropism using heavy-ion and laser microbeam. Heavy-ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporary suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy-ion broad-beam irradiation suppressed ${it de novo}$ expression of ${it INDOLE ACETIC ACID 5}$ gene, but not ${it MIZU-KUSSEI1}$ gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that ${it de novo}$ gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.



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