Interaction between nitrogen nutrients acquisition function and distribution of photosynthetic products

Oyama, Takuji*; Sueyoshi, Kuni*; Otake, Norikuni*; Ito, Sayuri*; Ishibashi, H.*; Hara, T.*; Kimura, T.*; Matsuhashi, Shimpei; Fujimaki, Shu; Suzui, Nobuo; Ishii, Satomi; Ishioka, Noriko; Watanabe, Satoshi; Kawachi, Naoki; Tsukamoto, Takashi

As nodulated leguminous crops grow, they assimilate both gaseous nitrogen and combined nitrogen. Combined N especially the NO form inhibits all phases of nodulation and N fixation process, although the mechanism have not been fully elucidated. Tungstate is a non-essential element for plant growth, which exerts harmaful effects on plants. When tungstate is applied to the plant medium, it competes with molybdate and inhibits some enzymes, which have a Mo cofactor in the active site such as nitrate reductase and nitrogenase. Application WO to the culuture medium diturbed nitrate transport to the shoot. In this report, WO was introduced into cut end of petiole of primary leaf and investigated that NO transport from root to shoot was inhibited or not.