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Report No.

Interaction between nitrogen nutrients acquisition function and distribution of photosynthetic products

Oyama, Takuji*; Sueyoshi, Kuni*; Otake, Norikuni*; Ito, Sayuri*; Ishibashi, H.*; Hara, T.*; Kimura, T.*; Matsuhashi, Shimpei; Fujimaki, Shu; Suzui, Nobuo; Ishii, Satomi; Ishioka, Noriko; Watanabe, Satoshi; Kawachi, Naoki; Tsukamoto, Takashi

As nodulated leguminous crops grow, they assimilate both gaseous nitrogen and combined nitrogen. Combined N especially the NO$$_{3}$$$$^{-}$$ form inhibits all phases of nodulation and N$$_{2}$$ fixation process, although the mechanism have not been fully elucidated. Tungstate is a non-essential element for plant growth, which exerts harmaful effects on plants. When tungstate is applied to the plant medium, it competes with molybdate and inhibits some enzymes, which have a Mo cofactor in the active site such as nitrate reductase and nitrogenase. Application WO$$_{4}$$$$^{2-}$$ to the culuture medium diturbed nitrate transport to the shoot. In this report, WO$$_{4}$$$$^{2-}$$ was introduced into cut end of petiole of primary leaf and investigated that $$^{13}$$NO$$_{3}$$$$^{-}$$ transport from root to shoot was inhibited or not.



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