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Silencing of CPD photolyase makes ${it Arabidopsis}$ hypersensitive and hypermutable in response to UV-B radiation

Yoshihara, Ryohei; Nakane, Chiyoko*; Sato, Ryohei*; Yasuda, Ai*; Takimoto, Koichi*

We generated a CPD photolyase knock-down in ${it Arabidopsis thaliana}$ by RNAi to investigate the role of CPD photorepair for protection of plants from solar UV. These knock-down lines exhibited hypersensitivity to UV-B and an increased occurrence of mutation induced by UV-B radiation compared with ${it Arabidopsis}$ proficient in CPD photolyase. These results indicate that CPD photoreactivation plays an important role for UV resistance of ${it Arabidopsis}$ and suppression of UV-induced mutagenesis.

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