A Photoelectron study for DNA and its damages in solution using monochromatic soft X-ray synchrotron radiation; Total and partial yields of photoelectrons for water and nucleotides

Ukai, Masatoshi*; Yokoya, Akinari; Nonaka, Yusuke*; Fujii, Kentaro; Saito, Yuji  

To substantiate the hydrated structure of DNA from the view point of the electronic properties, we developed a new spectroscopic technique of photoelectrons ejected from solutions dissolving DNA molecules using soft X-ray synchrotron radiation in combination with liquid jet in vacuum. In this paper, we present (1) the total photoelelctron yields for nucleotides (AMP, CMP, and GMP) in water solutions near nitrogen K-edge, which correspond to the X-ray absorption spectra (XANES) and (2) the partial yields of photoelectrons for liquid water molecules in the energy range around the oxygen K-edge, which are obtained by dispersing photoelectron energies. The experiments are carried out at the BL23SU in SPring-8, Japan. The liquid sample is maintained in the form of continuous micro-beam ( = 20 m) in vacuum and is intersected by a well focused nearly micro-beam of synchrotron-radiation of less than 50 m.