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The Effect of salt on the hierarchical structure of F-actin/polycation complexes; In situ observation by fluorescnece microscopy and small angle neutron scattering

Masui, Tomomi; Shikinaka, Kazuhiro*; Koizumi, Satoshi; Hashimoto, Takeji; Kakugo, Akira*; Gong, J.*; Kwon, H.*

We reconstituted in vitro model cytoskeleton using F-actin and polycation. We have investigated hierarchical structural change of F-actin/polycation complexes induced by salt quantitatively by fluorescence microscopy and small angle neutron scattering. Based on the obtained structural parameters, we will discuss dominant interactions which determine the structure.

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