A Method for the analysis of domain movements in large biomolecular complexes

巨大生体分子複合体のドメイン運動の解析手法

Poornam, G. P.*; 松本 淳; 石田 恒; Hayward, S.*

Poornam, G. P.*; Matsumoto, Atsushi; Ishida, Hisashi; Hayward, S.*

A new method for the analysis of domain movements in large, multichain, biomolecular complexes is presented. The method is applicable to any molecule for which two atomic structures are available that represent a conformational change indicating a possible domain movement. The method is blind to atomic bonding and atom type and can, therefore, be applied to biomolecular complexes containing different constituent molecules such as protein, RNA, or DNA. Here, we report on the application of the method to biomolecules covering a considerable size range: hemoglobin, liver alcohol dehydrogenase, S-Adenosylhomocysteine hydrolase, aspartate transcarbamylase, and the 70S ribosome. The results provide a depiction of the conformational change within each molecule that is easily understood, giving a perspective that is expected to lead to new insights.