SMAP1 that functions in 2,4-D response interacts with COP9 Signalosome

Nakasone, Akari; Kawai, Maki*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka

The () gene is recently identified as the factor that mediates the synthetic auxin 2,4-D response in root. SMAP1 has the conserved phenylalanine and asparagic acid (F/D) domain, which is highly conserved in both plants and animals. Introduction of to recovered 2,4-D sensitivity of while that of did not. Using anti-GFP-MicroBeads, the proteins that form complexes with SMAP1-GFP but not with SMAP1F/D-GFP were pulled down from the extract and identified by MS analysis. The identified peptide sequences indicated that SMAP1-GFP binds to COP9 Signalosome (CSN). The results implied that SMAP1 regulates 2,4-D response by interacting to CSN through F/D region.