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SMAP1 that functions in 2,4-D response interacts with COP9 Signalosome

Nakasone, Akari; Kawai, Maki*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka

The ${it Small Acidic Protein 1}$ (${it SMAP1}$) gene is recently identified as the factor that mediates the synthetic auxin 2,4-D response in ${it Arabidopsis}$ root. SMAP1 has the conserved phenylalanine and asparagic acid (F/D) domain, which is highly conserved in both plants and animals. Introduction of ${it SMAP1-GFP}$ to ${it aar1}$ recovered 2,4-D sensitivity of ${it aar1}$ while that of ${it SMAP1$Delta$F/D-GFP}$ did not. Using anti-GFP-MicroBeads, the proteins that form complexes with SMAP1-GFP but not with SMAP1$$Delta$$F/D-GFP were pulled down from the ${it Arabidopsis}$extract and identified by MS analysis. The identified peptide sequences indicated that SMAP1-GFP binds to COP9 Signalosome (CSN). The results implied that SMAP1 regulates 2,4-D response by interacting to CSN through F/D region.



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