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Report No.

Visualization of $$^{107}$$Cd translocation in Tobacco plants

Nakamura, Shinichi*; Suzui, Nobuo; Ishioka, Noriko; Kawachi, Naoki; Ito, Sayuri; Hattori, Hiroyuki*; Chino, Mitsuo*; Fujimaki, Shu

Tabacco plant is one of model plants which enable us to manipulate its gene expression, it is expected to create crop plants with low Cd content in the future. In this study, we visualized Cd dynamics in tobacco plant. Tobacco plants (${it Nicotiana tabacum}$) were grown hydroponically in a growth chamber where the growth conditions of plants were controlled completely for two weeks after sowing. After setting plants in the chamber, PETIS experiments were started by adding purified $$^{107}$$Cd in nutrient solutions. Time-series images of the $$^{107}$$Cd distribution were obtained with the PETIS apparatus. Each image was obtained every four minute for 36 hours. We succeeded to obtain fine serial images of Cd transport and accumulation in tobacco plants. Strong $$^{107}$$Cd signals were observed in the roots of tobacco plants. We also could see strong signals of $$^{107}$$Cd in the stems. In leaves, $$^{107}$$Cd signals were distributed thoroughly. The pattern of Cd signal distribution in the tobacco plants was similar to that in oilseed plants). These results demonstrated that dicotyledonous plants had the similar pattern of Cd distribution when plants were treated at low concentration of Cd.



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