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The Video-based quantitative evaluation of IR-induced effects on locomotory behavior in ${it Caenorhabditis elegans}$

Suzuki, Michiyo; Sakashita, Tetsuya; Hattori, Yuya; Yanase, Sumino*; Kikuchi, Masahiro; Funayama, Tomoo; Yokota, Yuichiro; Tsuji, Toshio*; Kobayashi, Yasuhiko

We reported an IR-induced reduction of locomotory rate of ${it Caenorhabditis elegans}$ in the absence of food. In the previous experiments, wild-type animals were irradiated with $$gamma$$-rays in the whole body, and measured the motility using "body bends" (the number of bends in the anterior body region at 20-s intervals). However, the IR-induced effects in the central and posterior body region were not evaluated by the body bends. In the present study, to investigate the IR-induced effects in more detail, we propose a novel method to evaluate the motility of the whole body using the video-based analysis.

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