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Report No.

The Production of $$^{13}$$N-labeled nitrogen gas tracer and the imaging of nitrogen fixation in soybean nodules

Ishii, Satomi; Suzui, Nobuo; Ito, Sayuri; Ishioka, Noriko; Kawachi, Naoki; Matsuhashi, Shimpei; Otake, Norikuni*; Oyama, Takuji*; Fujimaki, Shu

The nodule is a symbiotic organ of leguminous plants with rhizobium. Soybean plants utilize nitrogen (N) fixed by nodules from atmospheric N$$_{2}$$. Until now, $$^{15}$$N, a stable isotope, has long been used for studies of N$$_{2}$$ fixation. However, because this method is invasive, it has been difficult to analyze an instant response to an environmental (ex. temperature, light) changes. The purpose of our study is to image the N$$_{2}$$ fixation and analyze the kinetics quantitatively and noninvasively by using nitrogen gas labeled with $$^{13}$$N (half life: 10 min), a positron emitting isotope, and PETIS (positron-emitting tracer imaging system). Previously, we succeeded in producing $$^{13}$$N-labeled nitrogen gas However, it was required to purify the gas because it was likely to contain physiologically active $$^{13}$$N-labeled impurities. In this paper, we report development of a new method of production of pure $$^{13}$$N$$_{2}$$ and the imaging of nitrogen fixation.



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