Glutathione, administered to the roots, reduce Cd accumulation in the shoots of oilseed rape plants

Nakamura, Shinichi*; Suzui, Nobuo; Nagasaka, Toshinori*; Ito, Sayuri; Kawachi, Naoki; Ishioka, Noriko; Hattori, Hiroyuki*; Chino, Mitsuo*; Fujimaki, Shu

In our previous work, responses of GSH in sieve tubes to Cd treatment were investigated. These results suggested that GSH might be playing important roles in controlling Cd movement in plants. In this work, effects of GSH to Cd transport and accumulation were investigated when GSH was administered to specific organs. Plants were grown hydroponically in a green house and were treated with Cd and GSH. After 2D treatment, Cd content in their shoots and roots were measured. We employed PETIS (Position Emitting Tracer Imaging System) to visualize Cd movement in plants and analyzed the dynamics with the obtained image data. Cd content in the shoots was drastically reduced when their roots were treated with GSH. However, Cd content in the shoots increased slightly when sink-leaves were treated with GSH. Reduction of Cd transport and accumulation by GSH, administered to roots, was also confirmed by serial images of Cd movement, obtained by PETIS. Our results demonstrated that effects of GSH to Cd transport and accumulation in the plants were different in the organs where GSH was administered.