Analysis of mutational effects by radiation in higher plant

Yoshihara, Ryohei; Hase, Yoshihiro; Nozawa, Shigeki; Takimoto, Koichi*; Narumi, Issei

We irradiated carbon ion-beams (220-MeV C) and -rays to carrying gene to analyze mutational effects of these radiations. Our results suggested that -rays might induce smaller size of deletion mutation compared to 220-MeV C. The induction of oxidized guanine by these radiations was low in dry seeds because of low incidence of G:C to T:A and A:T to C:G transversion in irradiated group. -ray-induced mutation analysis in seedling was similar to that in dry seed, suggesting the mutational effect of radiation used here in higher plant might be different from that in other organisms.