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Report No.

Analysis of mutational effects by radiation in higher plant

Yoshihara, Ryohei; Hase, Yoshihiro; Nozawa, Shigeki; Takimoto, Koichi*; Narumi, Issei

We irradiated carbon ion-beams (220-MeV C) and $$gamma$$-rays to ${it Arabidopsis}$ carrying ${it rpsL}$ gene to analyze mutational effects of these radiations. Our results suggested that $$gamma$$-rays might induce smaller size of deletion mutation compared to 220-MeV C. The induction of oxidized guanine by these radiations was low in dry seeds because of low incidence of G:C to T:A and A:T to C:G transversion in irradiated group. $$gamma$$-ray-induced mutation analysis in ${it Arabidopsis}$ seedling was similar to that in dry seed, suggesting the mutational effect of radiation used here in higher plant might be different from that in other organisms.



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