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In situ experiment for redox buffer capacity in the subsurface environment

Amano, Yuki   ; Nanjo, Isao; Iwatsuki, Teruki  ; Sasaki, Yoshito ; Asano, Takahiro; Terashima, Motoki  ; Nagaoka, Toru*; Nakamura, Takamichi*; Yoshikawa, Hideki; Aoki, Kazuhiro 

In-situ experiment of redox reaction was conducted to assess the redox buffur capacity of water-rock-microbes system in the subsurface environment at 140 m depth of Horonobe Underground Research Laboratory. Dissolved oxygen was injected into a borehole, and the physico-chemical parameters (i.e. pH, ORP, DO, EC, etc.) and geochemical composition, microbial cell number, and microbial community structure were monitored continuously during the groundwater circulation. DO concentration could not detected after 10 hours from the injection. The concentration of Fe(II) was decreased with time, then the injected oxygen could be consumed by Fe(II). The rate of oxygen consumption during the experiment was 9.64 mg/L/day. The Eh value become stable at -208 mV after 5 days, then it showed that the reducing condition would be recovered quickly after closed condition.

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