Efficient induction of flower-color mutants by ion beam irradiation in Petunia seedlings treated with high sucrose concentration

高濃度ショ糖処理をしたペチュニア植物苗におけるイオンビーム照射による花色変異体の効率的誘導

長谷 純宏; 岡村 正愛*; 竹下 大学*; 鳴海 一成; 田中 淳

Hase, Yoshihiro; Okamura, Masachika*; Takeshita, Daigaku*; Narumi, Issei; Tanaka, Atsushi

We examined the effect of stress treatment on the frequency of flower-color mutants induced by ion beams. We found that petunia seedlings treated with 3% sucrose from 8 days after sowing accumulated significant amount of pigments within 4 days compared to non-treated control seedlings. The petunia seedlings treated with sucrose were exposed to 320-MeV carbon ions. The sucrose treatment did not affect the survival rate and seed fertility of the M1 plants. In the M2 lines obtained by self-pollination of individual M1 plants, chlorophyll mutants were obtained in both treated and non-treated groups with a similar frequency. Flower-color mutants such as magenta, purple and light pink were obtained from the original color of purplish blue. The frequency of flower-color mutants was significantly higher in sucrose-treated group than in non-treated group. These results suggest that the sucrose pretreatment specifically increases the frequency of flower-color mutation following ion beam irradiation.