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Mutation spectrum induced by $$gamma$$-rays and carbon ion beams in plant

Yoshihara, Ryohei; Hase, Yoshihiro; Nozawa, Shigeki; Narumi, Issei

$$gamma$$-rays and ion beams have been used for mutagenesis in mutation breeding, however, their mutational effects in plant were little known. Mutation breeding is one of the valuable breeding techniques that can produce various new phenotypes. It is necessary to investigate which types of mutation are induced by $$gamma$$-rays and ion beams for improvement of mutation breeding techniques. In this study, we analyzed mutation spectrum induced by $$gamma$$-rays and 220 MeV carbon ions to show the mutational effects of these radiations. Both radiation induced higher incidence of G:C to A:T transition and $$>$$ 2 bp deletions/insertions. The frequency of -2 and -1 frameshifts were higher in $$gamma$$-rays irradiated group. From our mutation spectrum analysis, it was shown that $$gamma$$-rays and carbon ions have different mutational effects. It would be effective for mutation breeding to use both $$gamma$$-rays and ion beams as the situation demands to induce different types of mutation.



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