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Report No.

Mutational effects of different LET radiations in ${it rpsL}$ transgenic ${it Arabidopsis}$

Yoshihara, Ryohei; Hase, Yoshihiro; Sato, Ryohei*; Takimoto, Koichi*; Narumi, Issei

In an effort to assess the characteristics of mutation induced by different LET radiation in higher plants, the mutational effects of carbon-ion beams and $$gamma$$-rays were investigated in ${it Arabidopsis}$. The ${it rpsL}$ transgenic ${it Arabidopsis}$ (Arabidopsis/rpsL) mutation detection system was adopted. Dry seeds of Arabidopsis/rpsL were irradiated with $$gamma$$-rays and 208-MeV carbon ions (208-MeV $$^{12}$$C$$^{5}$$), and the mutation frequency and mutation spectrum were examined. Our result suggested that 208-MeV $$^{12}$$C$$^{5}$$ and $$gamma$$-rays induced different mutational effects in ${it Arabidopsis}$ dry seed.



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