Biological imaging by soft X ray, 2; Imaging of organelles

Kado, Masataka; Ishino, Masahiko; Kishimoto, Maki; Shinohara, Kunio*; Tamotsu, Satoshi*; Yasuda, Keiko*; Yamamoto, Yoshimasa*; Kinjo, Yasuhito*

Contact soft X-ray microscopes with laser plasma X-ray sources are anticipated technology to be able to observe live hydrated biological cells with high spatial resolution. However there were no works reported which identified specific organelles clearly, because the obtained X-ray images are too complicated due to overlapping of cell structures. We have observed the same biological cells with both of a contact soft X-ray microscope and a confocal laser microscope and compared images obtained with both microscopes in order to identify organelles obtained with the soft X-ray microscope. As the results we succeeded to identify actin filaments and mitochondria clearly and found that the organelles obtained with the soft X-ray microscope were more detailed than those with the confocal laser microscope.