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Real-time imaging of zinc uptake and translocation in an intact plant using $$^{65}$$Zn

Suzui, Nobuo; Yamazaki, Haruaki; Kawachi, Naoki; Ishii, Satomi; Ishioka, Noriko; Fujimaki, Shu

Zinc is an essential element for plants. Real-time imaging of zinc dynamics has been a powerful tool for elucidating how plants regulate zinc uptake and translocation. In the past decade, we have employed a positron-emitting tracer imaging system (PETIS), which provides serial time-course images of the two-dimensional distribution of a radioisotope (e.g. $$^{11}$$C, $$^{13}$$N, $$^{52}$$Fe, $$^{64}$$Cu, $$^{107}$$Cd) in an intact plant without contact. Real-time imaging of zinc by PETIS has been conducted using $$^{62}$$Zn (half-life: 9.2 hours), which was produced by ourselves in the facility with a cyclotron. In this study, we demonstrate that real-time imaging of zinc is also possible using commercially available radioisotope, $$^{65}$$Zn (half-life: 244 days). The tracer solution containing $$^{65}$$Zn was fed to a rice plant and serial images of $$^{65}$$Zn distribution were successfully obtained by PETIS. Uptake kinetics (Km/V$$_{rm max}$$) and translocation velocity of zinc were determined from the image data. Furthermore, we observed zinc translocation for several weeks by taking advantage of the long half-life. These results indicate that $$^{65}$$Zn is widely useful for the analysis of zinc dynamics in plants.

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