Direct observation of P53 mediated bystander effect

Sato, Katsutoshi; Nishikino, Masaharu; Numasaki, Hodaka*; Kawachi, Tetsuya; Teshima, Teruki*; Nishimura, Hiroaki*; Matsuura, Nariaki*

Recently, the bystander effect induced by ionizing radiation has been reported by many researchers. Many signal transducers such as reactive oxygen species, nitric oxide, molecules involved in mitochondrial functions, and inflammatory cytokines were associated with the bystander effect. Whole phenomenon of the bystander effect must be clarified to understand the apparatus of the radiation effects on the cells. The laser plasma X-ray source has suitable features for this study. To elucidate the bystander effects, we have developed the microbeam system using a laser plasma X-ray, and the feasibilities in the radiation biology have been evaluated. In this research, we report that p53 function is associated with the bystander effect.