Crystal structure of a novel chitinase, a member of GH family 23, from the moderately thermophilic bacterium sp. A-471

Tamada, Taro; Okazaki, Nobuo; Ueda, Mitsuhiro*; Nakazawa, Masami*; Miyatake, Kazutaka*; Kuroki, Ryota

Crystal structure of a novel chitinase (Ra-ChiC), a member of GH family 23, from the moderately thermophilic bacterium sp. A-471 has been solved in the active site to 1.9 resolution. Crystal structure of Ra-ChiC was resemble to that of g-type lysozyme. It is well known that the residues involved in catalysis of the g-type lysozymes are Glu73, Asp90, and Asp101. The Glu73 (proton donor) in g-type lysozymes was conserved as Glu141 in the catalytic domain of Ra-ChiC.