-H2AX and phosphorylated ATM focus formation in cancer cells after laser plasma X irradiation

Sato, Katsutoshi; Nishikino, Masaharu; Okano, Yasuaki*; Oshima, Shinsuke*; Hasegawa, Noboru; Ishino, Masahiko; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Nishimura, Hiroaki*

Usefulness of laser-plasma X-ray pulse for medical and radiation biological studies was investigated and compared with that of conventional sources such as those obtained from a linear accelerator. A cell irradiation system was developed which uses Copper-K (8 keV) lines from an ultra-short high intensity laser to produce plasma. The absorbed dose of the 8 keV laser-plasma X-ray pulse was accurately estimated with Gafchromic EBT film. When the cells were irradiated with approximately 2 Gy of laser plasma X-ray, the circular regions on -H2AX positive cells could be clearly identified. Moreover, the number of -H2AX and phosphorylated ataxia telangiectasia mutated (ATM) foci induced by 8 keV laser plasma X-ray were comparable to those induced by 4MV X-ray. These results indicate that the laser plasma X-ray source can be useful for radiation biological studies.