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A New approach for building an atomic model from a three-dimensional electron microscopy data

電子顕微鏡3次元データから原子モデルを構築する新規手法の開発

松本 淳

Matsumoto, Atsushi

Recently, many three-dimensional structures of ribosome at various functional states have been determined by cryo-electron microscopy (cryo-EM) and reconstruction techniques. However, because of the low signal-to-noise ratio of the EM images, the resolution of the reconstructed 3D structures is low. The main goal of this study is to determine the positions of atoms in the ribosomes whose 3D structures have been determined by cryo-EM, and analyze the conformational differences between them at the atomic level. We make use of the fitting technique and the elastic network normal mode calculations for this purpose. In the fitting technique, the entire atomic structure of the ribosome is translated and rotated as a rigid body so that it overlaps a 3D EM structure at the maximum ratio. The atomic structure of the ribosome solved by X-ray crystallography is then computationally deformed, using a normal mode analysis of the elastic network model, so that it fits the EM structures better.

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