New strategy to enhance catalytic performance of whole cell biocatalysts harboring P450cam mutants

Mouri, Tsuyoshi; Kamiya, Noriho*; Goto, Masahiro*

In this paper, we have developed a new whole-cell biocatalytic system that affords the efficient microbial indigo production by cytochrome P450cam mutants (Y96F or F87W-Y96F) in the cells. The novel system is comprised of two types of P450cam mutants (Y96F or F87W-Y96F), putidaredoxin, putidaredoxin reductase, and bacterial glycerol dehydrogenase. The present whole-cell biocatalysts might be applicable to practical microbial indigo production.

Language	:	English
Journal	:	Biochemical Engineering Journal
Volume	:	53
Number	:	2
Pages	:	p.229 - 233
Publication Year/Month	:	2011/01
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Paper URL	:	https://doi.org/10.1016/j.bej.2010.09.016
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Keywords	:	no keyword
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Cooperating Institute	:	九州大学

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Registration No. : AA20100520
JAEA Abstracts No. : 39000152
Paper Submission No. : 8377

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