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Isolation and characterization of suppressors of the ${it early flowering 3}$ in ${it Arabidopsis thaliana}$

シロイヌナズナにおける${it early flowering 3}$の抑圧変異の単離と解析

夏井 悠*; Nefissi, R.*; 宮田 佳奈*; 小田 篤*; 長谷 純宏; 中川 繭*; 溝口 剛*

Natsui, Yu*; Nefissi, R.*; Miyata, Kana*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu*; Mizoguchi, Tsuyoshi*

EARLY FLOWERING 3(ELF3)は植物のサーカディアンリズムの維持に重要な役割を担うタンパクである。本研究では、EFL3によって制御される花成の分子機構を理解するため、${it elf3-101}$変異体の抑圧変異の単離を試みた。${it sel106}$及び${it sel105}$の2つの抑圧変異について詳しく調査した。遺伝子マッピング,発現解析及び塩基配列から${it sel106}$及び${it sel105}$はそれぞれ${it gi}$${it fca}$の新しいアリルであることがわかった。花成因子${it FLOWERING LOCUS T}$の制御に関して、${it elf3}$${it gi}$並びに${it elf3}$${it fca}$間の遺伝的関係についても調査した。単離した6つの${it elf3}$の抑圧変異は、花成因子の発現並びに開花時期に基づいて少なくとも4つのサブグループに分けられた。この分類方法は、未同定の抑圧変異の解析に有用である。

EARLY FLOWERING 3 (ELF3) is a circadian clock protein with a major role in maintaining circadian rhythms in plants. In this work, ${it elf3-101}$ was mutagenized by EMS in plants of the Lansberg ${it erecta}$ background to isolate suppressors of ${it elf3}$ and to understand the molecular mechanisms of flowering time controlled by ELF3. Two suppressors, ${it sel106}$ obtained from this screen and ${it sel5}$ from a precious study, were chosen for further analysis. Genetic mapping, gene expression analysis, and sequencing identified ${it sel106}$ and ${it sel5}$ as new alleles of ${it gi}$ and ${it fca}$, respectively. Genetic interactions between ${it elf3}$ and ${it gi}$ and between ${it elf3}$ and ${it fca}$ in the control of the floral activator ${it FLOWERING LOCUS T}$ were also investigated. Six suppressor of ${it elf3}$ were classified at least into four subgroups based on the expression of such floral regulators as GI, CO, FT, SVP, and FLC, and on their flowering times under LL, LD, and SD. This classification scheme is useful for the characterization of unidentified suppressor mutations.

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パーセンタイル:15.33

分野:Biotechnology & Applied Microbiology

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