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Report No.

Generation new ornamental plant varieties using ion beams

Affrida, A. H.*; Zaiton, A.*; Salahbiah, A. M.*; Shakinah, S.*; Yoshihara, Ryohei; Narumi, Issei; Hase, Yoshihiro; Ono, Yutaka

Chrysanthemum is one of the leading temperate flowers in Malaysia, and contributes 23% of the total value of cut flower production. The objectives of this study are to develop an effective method for mutation induction of local chrysanthemum varieties using ion beams and to determine the optimum dose for callus formation using petals. After 4 weeks, the percentage of survived petals of ${it Chrysanthemum morifolium}$ cv. Reagan Red decreased drastically at doses above 10 Gy. After 8 weeks, more cultures developed shoots or shoot buds. Irradiation dose of 15 Gy and higher was found to adversely affect the capability of the petal cultures to form shoots as the percentage of shoot regeneration were less than 5%.



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