Molecular analysis of carbon ion induced mutations in yeast cells

Shimizu, Kikuo*; Matsuo, Yoichiro*; Izumi, Yoshinobu*; Hase, Yoshihiro; Nozawa, Shigeki; Sakamoto, Ayako; Narumi, Issei

To elucidate the molecular mechanism of mutagenesis caused by ion beam irradiation in yeast, two mutant strains and which are deficient in mismatch repair mechanisms were used to measure mutation spectra. Several hot spots were found in the mutant, while mutations in the mutant were distributed evenly for base substitution except one hot spot at position 345. These results suggest that the incorporation of damaged nucleotides was not uniform in yeast cells.