Characterization of Xenopus laevis thrombocytic cells stimulated by thrombopoietin in liquid culture

液体培養中でトロンボポエチンによって刺激されるアフリカツメガエル栓球糸細胞の性状解析

田原 彩香*; 谷崎 祐太*; 奥井 武仁*; 目黒 瑞枝; 木下 紗也香*; 前川 峻*; 山内 志毅*; 下地 美也子*; 石田 貴子*; 永井 豊*; 黒木 良太; 加藤 尚志

Tahara, Ayaka*; Tanizaki, Yuta*; Okui, Takehito*; Meguro, Mizue; Kinoshita, Sayaka*; Maekawa, Shun*; Yamauchi, Motoki*; Shimoji, Miyako*; Ishida, Takako*; Nagai, Yutaka*; Kuroki, Ryota; Kato, Takashi

Most vertebrates have nucleated thrombocytes instead of platelets or megakaryocytes. African clawed frog Xenopus laevis, produces such thrombocytes in liver and spleen. We made a recombinant X.laevis thrombopoietin-like polypeptide (xlTPO) and found it had colony-forming activity in semi-solid assays of liver and spleen. Further studies focused on characterizing the thrombocytes and thrombocytic progenitors were performed to confirm whether xlTPO is a functional ortholog of mammalian TPO.