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Report No.

Study on bystander cell-killing effects induced by carbon-ion beams between normal fibroblasts and lung cancer cells

Ikeda, Hiroko; Yokota, Yuichiro; Funayama, Tomoo; Muto, Yasuko; Kanai, Tatsuaki*; Kobayashi, Yasuhiko

In this study, we investigated radiation-induced bystander effects between normal fibroblasts and cancer cells to elucidate the responses between normal tissues and tumor in heavy-ion radiotherapy. In our experiments, human lung normal fibroblasts WI-38 line and human lung cancer cells H1299/wtp53 line which is genetically modified to produce normal p53 proteins in their DNA damage response were used. Cells were irradiated with carbon-ion broad beams (LET = 108 keV/$$mu$$m, Dose = 0.5 Gy) or Co-60 $$gamma$$-rays (LET = 0.2 keV/$$mu$$m, Dose = 0.5 Gy), then survival rates of bystander cells after 6- or 24-hours co-culture with irradiated cells were calculated using colony formation assay. It was consequently found that survival rates of non-irradiated WI-38 cells increased when the cells were co-cultured with irradiated H1299/wtp53 cells. From this result, we conclude that some signals are released from irradiated H1299/wtp53 cells to promote cell adhesion and growth of bystander cells.



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