Chemical repair of damaged DNA by antioxidant, edaravone

Hata, Kuniki ; Urushibara, Ayumi; Yamashita, Shinichi; Shikazono, Naoya; Yokoya, Akinari; Muroya, Yusa*; Katsumura, Yosuke*

In the present study we verify the chemical repair property of an antioxidant, edaravone. At first, edaravone radical was measured by pulse radiolysis method to confirm that edaravone reacts efficiently with dGMP radical. In order to verify the chemical repair of radicals in DNA macromolecules by edaravone we irradiated -ray to aqueous solutions of plasmid DNA. In addition to strand breaks we quantified the chemical yields of AP sites and base lesions using base excision repair enzymes. Production of AP sites and base lesions were more significantly inhibited than that of strand breaks by addition of edaravone. This result indicates that edaravone can chemically repair DNA radicals resulting in AP sites or base lesions on DNA.