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The Analysis of boron micro-distribution in 9L gliosarmoma cells and HUVEC cells using PIXE and PIGE

Endo, Kiyoshi*; Shibata, Yasushi*; Yamamoto, Tetsuya*; Nakai, Kei*; Matsumura, Akira*; Sato, Takahiro; Yokoyama, Akihito; Koka, Masashi; Okubo, Takeru; Yamazaki, Akiyoshi; Ishii, Yasuyuki; Kamiya, Tomihiro; Ishii, Keizo*

Micro particle-induced X-ray emission (micro-PIXE) was applied to determine the inter- and intracellular distribution of boron-10 ($$^{10}$$B) in tumor cells. The peak $$^{10}$$B atom measurement was large on the measurement spectrum in comparison to the circumference background. It was possible to confirm that $$^{10}$$B atoms were measured. But, the intracellular micro-distribution of boron could not be clearly detected in this analysis. Improvements are therefore necessary in the technical methods of cell fixation, while the micro-PIXE and PIGE analyzing system also needs to be further upgraded.

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