Feasibility study for the quantification of total protein content by multiple prompt -ray analysis

Toh, Yosuke   ; Murakami, Yukihiro*; Furutaka, Kazuyoshi  ; Kimura, Atsushi   ; Koizumi, Mitsuo ; Hara, Kaoru; Kin, Tadahiro; Nakamura, Shoji  ; Harada, Hideo   

By applying the multiple -ray detection method to prompt -ray analysis, called MPGA (multiple prompt -ray analysis), the interference from hydrogen contained in a sample can be reduced. Therefore, this method can improve the signal-to-noise ratio, and provide a rapid screening method of toxic elements. We developed a neutron beam line and a detector system for MPGA, which consists of eight Clover Ge detectors, was constructed at the cold neutron guide C2-3-2 of JRR-3. Protein is an important nutrient in foods. The classical total protein analysis method is the Kjeldahl technique, which is time-consuming and inconvenient. As a convenient method to quantify total protein content in biological samples, the feasibility of application of MPGA to the quantification was studied. The results obtained with reference materials are in good agreement with the certified value. MPGA provides a tool for the rapid and non-destructive quantification of total protein content.