Analysis of mutated region on DNA in ion beam-induced UVB tolerant or sensitive rice mutant

Takano, Nao*; Takahashi, Yuko*; Yamamoto, Mitsuru*; Teranishi, Mika*; Hase, Yoshihiro; Sakamoto, Ayako; Tanaka, Atsushi; Hidema, Jun*

UVB radiation can affect the plant growth and development. The aim of this study is identify new factor(s) related to UVB tolerance in plants by bioengineering or breeding programs. To produce UVB tolerant or sensitive mutants, dry seeds of Japanese rice cultivar Sasanishiki were exposed with carbon ion beams, and two UVB tolerant and three UVB sensitive mutants were isolated from the offspring of irradiated plants. By analysis of comparative genomic hybridization and sequencing of bacterial artificial chromosome library, we identified the mutated regions in a UVB tolerant mutant and a UVB sensitive mutant .