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Report No.

Stability evaluation of mutant lines induced by ion beam breeding in petunia

Okamura, Masachika*; Hase, Yoshihiro; Onishi, Noboru*; Narumi, Issei; Tanaka, Atsushi

In this report, we describe results of the stability examination of the mutated characteristics and the trial to develop superior breeding lines for the commercial production of petunia new varieties. Flower color mutants obtained in M2 generation from the original blue color petunia BBss11 include magenta, purple, purple vein, light pink, white and burgundy. We collected more than a hundred M3 seeds from each of the M2 plants and examined their segregation. Of the 12 mutant lines tested, six lines clearly showed the stability in flower color, flower shape and plant morphology. Three M3 lines from white-colored M2 plants showed good stability in flower color, flower size and growth habit. They are expected to be superior parents for new varieties of petunia.



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