Involvement of universal conserved genes, and orthologs, in DNA repair of

Onodera, Takefumi; Sato, Katsuya; Narumi, Issei; Ota, Toshihiro*

In this study, we generated and disruptant strains and investigated the disruption effect to DNA damaging agents in an effort to gain insight into the role of DrygjD and DryeaZ proteins in . We successfully constructed disruptant, disruptant and double-disruptant strains. All the disruptant strains did not exhibit the delayed growth compared to the wild-type strain, clearly indicating that neither DrygjD nor DryeaZ protein is essential for cell viability. These gene disruptant strains exhibited extremely sensitivity to mitomycin C (MMC), which causes inter- and intrastrand DNA crosslinking, suggesting that DrygjD and DryeaZ proteins are involved in the DNA repair mechanism and especially play a critical role in MMC-induced DNA damage in .