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セルラーゼを高発現する酵母の蛍光活性化セルソーターによる繰り返し選抜

Repeated FACS-based screening for yeast strain highly expressing cellulase

仁宮 一章*; 曽田 裕司*; 佐藤 勝也; 鳴海 一成; 清水 宣明*

Ninomiya, Kazuaki*; Soda, Hiroshi*; Sato, Katsuya; Narumi, Issei; Shimizu, Nobuaki*

By using immunocytochemistry and fluorescence-activated cell sorter (FACS), yeast population highly expressing cellulase on the cell surface was enriched from the mutant population prepared by irradiation with carbon ion beams (220 MeV $$^{12}$$C$$^{5+}$$, 100 Gy). Repeated FACS after irradiation with carbon ion beams was effective for selecting yeast population of interest.

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