Development of the fluorescent probe for capillary electrophoresis aiming at simple and safe analysis of actinide ions

Sato, Yoshiyuki; Saito, Shingo*; Haraga, Tomoko   ; Nakano, Yuta*; Kameo, Yutaka ; Takahashi, Kuniaki ; Shibukawa, Masami*

In this research, capillary electrophoresis - laser induced fluorescence detecting method (CE-LIF) for the simple screening method of actinide (An) ions was studied. In order to detect An ion by CE-LIF, eight kinds of fluorescent probes were synthesized which have acyclic or macrocyclic 4-8 denate chelating group. An ions were derivatized to from a strongly fluorescent complexes with the probe to suppress the quenching of the ligand - centered emission. These An complexes were applied to CE-LIF, and it looked for the probe suitable for detection of An ions. As a result, Th, U and Np were detectable with octadenate probe, tetradenate probe and hexa and hepta probe respectively. Am was detectable with all the probes except tetradenate probe. An ions were separated from coexistence metal ions, such as Ca, Fe, Al and lanthanide ions, and a lower detection limits of tens ppt revel for An ions were successfully.