Neurocytotoxic effects of microbeam irradiation of heavy carbon-ions on the developing brain measured in vivo using medaka (), a vertebrate model

Yasuda, Takako*; Oda, Shoji*; Hibi, Yusuke*; Urushihara, Yusuke*; Mitani, Hiroshi*; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko; Ikeda, Hiroko; Kobayashi, Yasuhiko

Using heavy-ion microbeam system of JAEA-Takasaki, we irradiated brain of the embryos of the Medaka with carbon ion microbeam. After irradiation, inductions of apoptosis were observed using whole-mount staining of acridine orange. In targeted position of the brain, we observed the induction of radiation induced apoptosis, indicating that the system can irradiate specific region of the brain of the Medaka embryo.