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Exploration of radiation response sites for the salt chemotaxis learning of ${it C. elegans}$ using heavy-ion microbeam

Sakashita, Tetsuya; Suzuki, Michiyo; Hattori, Yuya; Ikeda, Hiroko; Muto, Yasuko; Yokota, Yuichiro; Funayama, Tomoo; Hamada, Nobuyuki*; Fukamoto, Kana*; Kobayashi, Yasuhiko

We have no direct evidence for the interaction of ionizing radiation with the central neuronal tissue (nerve ring) of the nervous system in ${it C. elegans}$. Localized ionizing irradiation is useful to analyze radiation effects at a cellular or tissue level. Thus, to investigate the effects on the nerve ring, we used the heavy-ion microbeam system installed at the Takasaki Ion accelerators for Advanced Radiation Application of JAEA. To achieve during-learning irradiation, we have developed the live-targeting system for non-paralyzed ${it C. elegans}$ using the micro-total analysis systems to restrict their motion, and this will be discussed.

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