An Improved system for shoot regeneration from stem explants of Lombardy poplar ( L. var. Koehne)

Biswas, K.; Mori, Takeshi*; Kogawara, Satoshi*; Hase, Yoshihiro; Narumi, Issei; Ono, Yutaka

We developed a system for the regeneration of Lombardy poplar ( L. var. ) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashige and Skoog (MS) medium supplemented with 0.1 mg/L indole-3-acetic acid and 0.5 mg/L benzylaminopurine without undergoing callus formation. About 80% of the regenerated shoots developed roots on MS medium supplemented with 0.5 mg/L indole-3-butyric acid and 0.02 mg/L 1-naphthylacetic acid. The protocol presented here is simple and economical because it does not rely on pre-incubation in callus induction medium or repeated subculture in shoot induction medium containing -zeatin, an expensive substance. The regeneration system presented here could be used for evaluation of radiation sensitivity for Lombardy poplar tissues.